Annotation Guide
- Assignments are based on compositions, taking into account biosynthetic considerations.
- Alternative sequences are possible. In such cases ES-MS/MS will differentiate alternative possibilities.
Pictures and images are mostly home-made, but a number have been grabbed and modified from random websites - many thanks to the original authors.
Complete Annotation Guide
Protocols
- Extraction of tissue
- Reduction/carboxymethylation
- Tryptic digestion
- PNGaseF digestion
- Sep-Pak separation of N-glycans O-glycopeptides/peptides
- Reductive elimination of O-glycans
- Dowex purification of O-glycans
- Permethylation of N- and O-glycans
- MALDI-TOF MS of permethylated N- and O-glycans Annotations
Complete protocols for both Cell and Tissue extraction
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Monosaccharide Key
Symbol Nomenclature for Representation of Glycan Structure
(May 2004, Nomenclature Committee,
Consortium for Functional Glycomics)
Full documentation available at:
http://glycomics.scripps.edu/CFGnomenclature.pdf |
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