GLYCAN ARRAY SYNTHESIS CORE (D)
Overview
This Core is producing a library
of oligosaccharides for use by Participating Investigators
and other Cores. These compounds represent a diverse range of natural oligosaccharide sequences of
glycoproteins and glycolipids with linker arms compatible for use as monovalent oligosaccharides and
for coupling to biotin, polymers or column supports. This Core is working with the
Protein-Glycan Interaction Core (H) to create a glycan array,
a library of oligosaccharides for the rapid screening of the carbohydrate binding specificity
of glycan-binding proteins (GBPs). This Core is also producing recombinant proteins
including glycosyltransferases and GBPs. Information relating to the production of each
material, including novel synthetic procedures, is being deposited in the
Central Database. Available materials are posted on
the website and are distributed to investigators both within and outside of the Consortium upon
approval by the Steering Committee.
Specific Objectives
- Produce a diverse range of natural oligosaccharide sequences of glycoproteins and
glycolipids with linker arms compatible for use as monovalent oligosaccharides and for
coupling to proteins or solid supports
- Work with the Protein-Glycan Interaction Core (H) to create
a glycan array of oligosaccharides for rapid screening of the binding specificity of GBPs
- Conduct custom synthesis of oligosaccharides by chemoenzymatic or one pot chemical synthesis
methods
- Construct multivalent oligosaccharide probes labeled with biotin or fluorescein or other
labels for detecting cell surface GBPs
- Produce recombinant glycosyltransferases and other enzymes for carbohydrate synthesis
- Produce recombinant GBPs for determination of binding specificity by the
Protein-Glycan Interaction Core (H), crystallography, and
production of antibodies
Core D Team
- Jim Paulson and Nicolai Bovin, Coordinators
- Nahid Razi, Director
- Kenneth Weichert, Research Assistant, Chemistry and Enzymology
- Oren Berger, Research Assistant, Chemistry
- TBA, Research Assistant, Protein Expression and Purification
- Leila Khajavi, Research Assistant, Molecular Biology and Protein Expression
- Xiaofei Liu, Research Assistant, Molecular Biology and Protein Expression
- Ogie Bohorov, Research Specialist, Enzymology
- Julia Busch, Research Assistant, Glycan Arrays
- Ryan McBride, Research Technician, Glycan Arrays
- TBA, Administrative Assistant
|
Last Updated Saturday, 21-Jun-2008 07:56:29 EDT. Please contact us
with comments/questions.
Top of page
|
