Functional Glycomics homepage

ENZYMES



One activity of the CFG's Glycan Array Synthesis Core (Core D) was to produce glycosyltransferases for use in chemo-enzymatic synthesis of glycans. Several useful glycosyltransferases were produced with the intention of distributing them to investigators through the Reagent Bank. However, distribution of these enzymes was restricted by the institutions that originally provided them. TSRI worked with each institution to remove these restrictions. Permission was obtained to distribute the enzymes listed below, which may be requested using the standard CFG resource request form. To cover the cost of distributing materials, investigators will be requested to pay a $250.00 handling fee plus shipping costs for each resource request. A request can include more than one reagent.

Glycosyltransferases available for distribution
Enzyme Quantity
beta 1-3GlcNAcT (lgtA) 1 Unit/1 ml/vial
UDP Glc/GalE (GalE) 5 Unit/1 ml/vial
alpha 1-4 Gal T (lgtC) 11 Unit/1 ml/vial
alpha 1-3GalNAcT (Forsmann) 6.8 Unit/1 ml/vial
CMP-NeuAc synthetase 1.2 Unit/1 ml/vial
alpha2-3SiaT/CMP Synt (ST3) 1 Unit/1 ml/vial
alpha2,3 SiaT/alpha2,8SiaT 0.5 Unit/1 ml/vial




Glycosyltransferases used by Core D to synthesize the glycan library.
The table below lists the enzymes used by Core D for synthesis of glycans for the glycan array library. Blue highlighting on 'Entry' numbers signifies enzymes that have been produced by Core D for the reagent bank; you may request these enzymes through the normal request process, and approved requests will be filled when distribution rights have been obtained. Further expansion of the reagent bank has been suspended due to lack of resources. If you wish to request the expression systems or cDNAs of any of the enzymes for use in your own laboratory, please contact the contributor directly to obtain permission. To cover the cost of distributing materials, investigators will be requested to pay a $250.00 handling fee plus shipping costs for each resource request. A request can include more than one reagent.
Entry Name (abbr) Organism Acceptor substrate(s) Product Expression system Date recieved Source/ Contact Source/ Institution Right of TSRI to Transfer Commercial Source
1 KSGal6ST Human -Gal- -(6SO3)Gal-R Mammalian cells Jul- 05 Michiko Fukuda Burnham No  
2 hcGlcNAc6ST Human GlcNAc-R (6SO3)GlcNAc-R Mammalian cells Jul- 05 Michiko Fukuda Burnham No  
3 a1-4GnT Human Galb-R GlcNAca1-4Galb-R Mammalian cells May-05 Minoru Fukuda Burnham No  
4 a2-3SiaT (pST3Gal I ) Porcine Galb1-3GalNAc-R NeuAca2-3Galb1-3GalNAc-R Bacculovirus May-95 Shawn DeFrees Cytel/Neose TBD Calbiochem
5 a1-2FucT (FUT2) Human Gal-R Fuca1-2Gal-R Bacculovirus May-95 Shawn DeFrees Cytel/Neose No  
6 a1-3FucT (FUT3) Human Galb1-4GlcNAc-R Galb1-4(Fuca1-3)GlcNAc-R Bacculovirus May-95 Shawn DeFrees Cytel/Neose No  
7 a1-3FucT (FUT4) Human Galb1-4GlcNAc-R Galb1-4(Fuca1-3)GlcNAc-R Bacculovirus May-95 Shawn DeFrees Cytel/Neose No  
8 a1-3/4FucT (FUT5) Human Galb1-4GlcNAc-R Galb1-4(Fuca1-3)GlcNAc-R Bacculovirus May-95 Shawn DeFrees Cytel/Neose No  
9 a1-3FucT (FUT6) Human NeuAca2-3Galb1-4GlcNAc-R NeuAca2-3Galb1-4(Fuca1-3)GlcNAc-R Bacculovirus May-95 Shawn DeFrees Cytel/Neose No  
10 a1-3FucT(FUT7) Human NeuAca2-3Galb1-4GlcNAc-R NeuAca2-3Galb1-4(Fuca1-3)GlcNAc-R Bacculovirus May-95 Shawn DeFrees Cytel/Neose No  
11 b1-4GalNAcT (mutant) Bovine GlcNAc-R GalNAcb1-4GlcNAc-R Bacteria Oct-02 Pradman Qasba National Cancer Institute (NCI) No  
12 b1-3GlcNAc T(lgtA) N. meningitidis Galb1-4-R GlcNAcb1-3Galb1-4-R Bacteria Apr-99 Warren Wakarchuk Rockefeller University Yes  
13 UDP Glc/GalE (GalE) P.aeruginosa UDP-Glc UDP-Gal Bacteria Apr-99 Warren Wakarchuk National Research Council of Canada Yes  
14 b1,3GalT(cgtB) C. jejuni GalNAcb1-4(NeuaAca2-3)GalbR (GM2) Galb1-3GalNAcb1-4(NeuAca2-3)Galb-R  (GM1) Bacteria Dec-02 Warren Wakarchuk National Research Council of Canada Pending  
15 a1-4GalT/UDPGalE (lgtC) N. meningitidis Galb1-4-R Gala1-4Galb1-4-R (p antigen) Bacteria May-02 Warren Wakarchuk Rockefeller University Yes  
16 b1-4GalT/UDPGalE (lgtB) N.meningitidis GlcNAc-R Galb1-4GlcNAc-R Bacteria Apr-99 Warren Wakarchuk Rockefeller University Yes  
17 b1-4GalNAcT (cgtA) C jejuni NeuAca2-3Galb-R GalNAcb1-4(NeuAca2-3)Galb-R (GM2) Bacteria May-02 Warren Wakarchuk National Research Council of Canada Pending  
18 a2-3SiaT/CMP Synt (ST3) N. meningitidis Gal-R NeuAca2-3Gal-R Bacteria Feb-99 Warren Wakarchuk National Research Council of Canada Yes  
19 a2-3SiaT/a2-8SiaT (cstII) C. jejuni NeuAca2-3Gal-R NeuAca2-8NeuAca2-3Gal-R Bacteria May-02 Warren Wakarchuk National Research Council of Canada Yes  
20 a1-3GalNAcT (Forsmann) Pasteurella GalNAcb1-3Gala1-4Galb1-4-R GalNAca1-3GalNAcb1-3Gala1-4Galb1-4-R (Forsmann) Bacteria Apr-07 Warren Wakarchuk National Research Council of Canada Yes  
21 b1-3GalT(GalT5) Human GlcNAc-R Galb1-3GlcNAc-R(type 1) Bacculovirus Nov-99 Shawn DeFrees Neose Technologies, Inc. No  
22 a2-3SiaT (rST3Gal III) Rat Galb1-4GlcNAc-R NeuAca2-3Galb1-4GlcNAc-R Aspergillus niger Feb-00 Shawn DeFrees Neose Technologies, Inc. TBD Calbiochem
23 UDP-GlcNAc/GalNAc-4-E Rat UDP-Glc/GlcNAc UDP-Gal/GalNAc Bacteria Mar-01 Shawn DeFrees Neose Technologies, Inc. Yes  
24 b1-6GlcNAc T(C2GnT) Human (Galb1-3)GalNAc-R GlcNAcb1-6(Galb1-3)GalNAc-R (Core 2) Mammalian cells Jun-05 Richard Cummings Oklahoma University No  
25 a1-3FucT(FUT8) Human GlcNAc-R Fuca1-6GlcNAc  (N-linked core fucose) Bacculovirus May-06 Naoyuki Taniguchi Osaka University Yes  
26 chST6GalNAcI Chicken GalNAcaThr NeuAca2-6GalNAcaThr Bacculovirus Mar-99 Shuichi Tsuji Riken Yes  
27 hST6GalNAc I Human GalNAc-R NeuAca2-6GalNAc-R Bacculovirus Apr-04 Celso Reis University of Porto No  
28 hST6GalI Human Galb1-4GlcNAc-R NeuAca2-6Galb1-4GlcNAc-R Bacculovirus/ mamalian cells Dec-99 James Paulson TSRI Yes Calbiochem
29 ST6GalT Pasteurella Gal-R NeuAca2-6Gal-R Bacteria Jun-05 Xi Chen University of California, Davis No Japan Tobacco
30 b1-3GalT Drosophila GalNAc-R Galb1-3GalNAc-R(core1) Bacculovirus Oct-05 Thierry Hennet University of Zurich No  
31 b1-3GalNACT/ UDPGalNAcE (lgtD) P. shigelloides Gala1-4Galb1-4-R GalNAcb1-3Gala1-4Galb1-4-R Bacteria Jun-05 Peng 'George' Wang Wayne State University TBD  
32 a1-3GalT (BraGalT1) Bovine Galb1-4GlcNAc-R Gala1-3Galb1-4GlcNAc-R (Galili) Bacteria Feb-99 Peng 'George' Wang Wayne State University TBD  
33 CMP-NeuAc synthetase N. meningitidis CTP and NeuAc Bacteria Warren Wakarchuk NRC Yes  

Request Resources

More Information

Methods and Data