| GLYCO-GENE CHIP
Investigator's Responsibility
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RNA Sample Requirements:
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1. Ideally, 5 micrograms of
total RNA should be provided at a concentration of 1 microgram per
microliter in water (this amount allows for sample quality control, labeling, and any necessary re-preps). For most studies, 0.5 to 1.0 micrograms will be sufficient for labeling. However, as little as 10 - 100 nanograms may be
sufficient with an alternate amplification protocol. Please see the Core E protocols or contact the Director
for further information regarding RNA samples that are less than 5 micrograms.
2. RNA must be purified using an RNeasy column
(Qiagen) and
RNA Sample Preparation Protocol.
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Experimental Design: |
Because it is not practical to develop one
experimental design that is appropriate for all microarray
experiments, several factors should be considered when designing
microarray experiments for measuring gene expression changes in
biological systems. Please see the
PDF file for more information.
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Shipping Instructions: |
1. Package RNA samples on dry ice, or for shipment at room temperature, follow the protocol at http://www.biomatrica.com/product/rnaProtocol.html.
2. Send RNA samples by an overnight service [e.g. Federal Express, UPS, DHL, etc.]
deliver to the address below.
Do not have samples arrive on a Saturday or Sunday.
Note: No overnight delivery is needed if sending samples at room temperature . Again no weekend deliveries.
3. Call or send an e-mail to the Director and
provide the airbill/tracking number.
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Core's Responsibility
Request Glyco-gene Chip Analysis or Glyco-gene Chips
Shipping Address
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