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Mammalian Glycan Array
The Consortium for Functional Glycomics' printed mammalian glycan microarray format has replaced the earlier ELISA-based microplate array for routine screening of glycan-binding protein (GBP) specificity. A growing library of natural and synthetic mammalian glycans with amino linkers is printed onto N-hydroxysuccinimide (NHS)-activated glass microscope slides (SCHOTT Nexterion) to form covalent amide linkages in this printed array format.
The current mammalian array (version 4) has 442 glycan targets.
Pathogen Glycan Array
In September 2008, the first version of a pathogen glycan array was made available for screening. This array employs polysaccharides derived from dozens of Gram-negative bacteria, largely provided by Dr. Yuri Knirel's group at the ND Zelinsky Institute of Organic Chemistry in Moscow, Russia. The current pathogen glycan array (version 1.0) has 96 glycan targets representing E. coli, Proteus mirabilis, Pseudomonas aeruginosa, Providencia alcalifaciens, Providencia rustigianii, Providencia stuartii, Shigella boydii, and Shigella dysennteriae. Pathogen array v1.0 was engineered by coupling these bacterial polysaccharides to NHS-activated glass slides, the common platform utilized for the currently available mammalian glycan array.
Glycan Array Screening at Core H
The Protein-Glycan Interaction Core (H) analyzes investigator-generated lectins, antibodies, antisera, microorganisms, or suspected GBPs of human, animal, and microbial origins on the mammalian and pathogen glycan microarrays. The protocols used for these analyses require fluorescent reagents for detecting primary binding to the glycans on the array.
