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Mammalian Glycan Array
The Consortium for Functional Glycomics' printed mammalian glycan microarray format has replaced the earlier ELISA-based microplate array for routine screening of glycan-binding protein (GBP) specificity. The comparison of "ELISA" and "Printed" arrays requested by the Steering Committee to validate the printed array can be viewed here.
A growing library of natural and synthetic mammalian glycans with amino linkers is printed onto N-hydroxysuccinimide (NHS)-activated glass microscope slides (SCHOTT Nexterion) to form covalent amide linkages in this printed array format.
The current mammalian array (version 4.1) has 465 glycan targets.
Pathogen Glycan Array
After three years of careful planning and significant input from Participating Investigators, the CFG announced the release of a pathogen glycan array in September 2008. However, before any screening requests could be processed, funding for the pathogen glycan array was cut and the CFG was ordered to cease development and use of this array immediately. The CFG is currently seeking alternative funding for the pathogen glycan array. For updates and more information, please see the pathogen glycan array status page.
Glycan Array Screening at Core H
The Protein-Glycan Interaction Core (H) analyzes investigator-generated lectins, antibodies, antisera, microorganisms, or suspected GBPs of human, animal, and microbial origins on the mammalian glycan
array. The protocols used for these analyses require fluorescent reagents for detecting primary binding to the glycans on the array.
