Glycan Array Experiment: primscreen_PA_v1_264_12072005

Glycan Array ID : primscreen_PA_v1_264_12072005
Sample Name : Influenza virus A/New_York/1/1918 (H1N1) Asp190Glu mutant
Sample Category : Other
Investigator: : James Paulson
Download Data:
File: 7) A_New York_1_1918_ASP190Glu formatted Core H (2).xls
Conclusive
Sample Information:
Name : Influenza virus A/New_York/1/1918 (H1N1) Asp190Glu mutant
Complete Name : Influenza virus A/New_York/1/1918 (H1N1) Hemagglutinin Asp190Glu mutant
Family : Other
Sub Family : Viral lectin
Gene Symbol : Information not entered/not applicable.
Synonyms : Information not entered/not applicable.
Species Common Name: : Information not entered/not applicable.
Species Scientific Name : Influenza A virus (A/New_York/1/1918 (H1N1))
Estimated Purity : His Tag fusion
Mutation/Chimera : Asp190Glu
Entry Date [yyyy-mm-dd] : 2006-03-10
Status : Public
Primary Sequence :
MEARLLVLLCAFAATNADTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCKLKGIAPLQLGKCNIAGWLLGNPECDLLLTASSWSYIVETSNSENGTCYPGDFIDYEELREQLSSVSSFEKFEIFPKTSSWPNHETTKGVTAACSYAGASSFYRNLLWLTKKGSSYPKLSKSYVNNKGKEVLVLWGVHHPPTGTEQQSLYQNADAYVSVGSSKYNRRFTPEIAARPKVRGQAGRMNYYWTLLEPGDTITFEATGNLIAPWYAFALNRGSGSGIITSDAPVHDCNTKCQTPHGAINSSLPFQNIHPVTIGECPKYVRSTKLRMATGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAIDGITNKVNSVIEKMNTQ
Comments :
Information not entered/not applicable.
Known Sites of Expression :
Information not entered/not applicable.
External References :
Swissprot : Information not entered/not applicable.
Genbank : Information not entered/not applicable.
Experiment Information:
Status: : Public
Title/Project Description: : Two projects on influenza virus receptors
Associated Resource Requests: : cfg_rRequest_451
Glycan Array Version: : PA_v1
Protocol Used: : Information not entered/not applicable.
Replicate Number: : 1
Result Nature: : Data
Protein/Sample Concentration: : Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]: : 2005-12-05
Supplying Investigator : James Paulson
Specific Assay Information : Glycan receptor specificities of viral surface hemagglutinin glycoproteins in H1 and H3 avian and human Influenza viruses..Hemagglutinin (HA)-antibody pre-complexes were prepared by mixing HA (1mg/ml, 15 <font face="symbol">m</font>l), mouse anti-penta-His-Alexa Fluor488 (0.2mg/m, 35<font face="symbol">m</font>l, Qiagen, Valencia, CA) and anti-mouse-IgG-Alexa Fluor488 (2.3<font face="symbol">m</font>l, 2mg/ml) in a molar ratio of 4:2:1, respectively. Mixtures were incubated for 15 min on ice, diluted with PBS buffer containing 3 percent (w/v) bovine serum albumin (50<font face="symbol">m</font>l) and incubated on the glycan array slide under a microscope cover-glass in a humidified chamber for 30 min. Slides were subsequently washed by successive rinses in PBS with 0.05 percent Tween-20, PBS, and deionized water, then immediately subjected to imaging analysis..Storage: 4 degrees C
Annotation : Information not entered/not applicable.
Protein Sample Analyzed : Influenza virus A/New_York/1/1918 (H1N1) Asp190Glu mutant