Glycan Array Experiment: primscreen_PA_v1_323_12072005
Glycan Array ID
:
primscreen_PA_v1_323_12072005
Sample Name
:
Influenza virus A/Duck/Alberta/35/1976 (H1N1) Gly225Asp mutant
Sample Category
:
Other
Investigator:
:
James Paulson
Download Data:
Sample Information:
Name
:
Influenza virus A/Duck/Alberta/35/1976 (H1N1) Gly225Asp mutant
Complete Name
:
Influenza virus A/Duck/Alberta/35/1976 (H1N1) Hemagglutinin Gly225Asp mutant
Family
:
Other
Sub Family
:
Viral lectin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Influenza A virus (A/Duck/Alberta/35/1976 (H1N1))
Estimated Purity
:
None
Mutation/Chimera
:
Gly225Asp
Entry Date [yyyy-mm-dd]
:
2006-03-08
Status
:
Public
Primary Sequence
:
MEAKLFVLFCTFTVLKADTICVGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCSLNGIAPLQLGKCNVAGWLLGNPECDLLLTANSWSYIIETSNSENGTCYPGEFIDYEELREQLSSISSFEKFEIFPKASSWPNHETTKGVTAACSYSGASSFYRNLLWITKKGTSYPKLSKSYTNNKGKEVLVLWGVHHPPSVSEQQSLYQNADAYVSVGSSKYNRRFAPEIAARPEVRDQAGRMNYYWTLLDQGDTITFEATGNLIAPWYAFALNKGSDSGIITSDAPVHNCDTRCQTPHGALNSSLPFQNVHPITIGECPKYVKSTKLRMATGLRNVPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAIDGITSKVNSVIEKMNTQFTAVGKEFNNLERRIENLNKKVDDGFLDVWTYNAELLVLLENERTLDFHDSNVRNLYEKVKSQLRNNAKEIGNGCFEFYHKCDDECMESVKNGTYDYPKYSEESKLNREEIDGVKLESMGVYQILAIYSTVASSLVLLVSWGAISFWMCSNGSLQCRICINGSL
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Information not entered/not applicable.
External References :
Swissprot
:
Information not entered/not applicable.
Genbank
:
Information not entered/not applicable.
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Two projects on influenza virus receptors
Associated Resource Requests:
:
cfg_rRequest_451
Glycan Array Version:
:
PA_v1
Protocol Used:
:
Information not entered/not applicable.
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2005-12-05
Supplying Investigator
:
James Paulson
Specific Assay Information
:
Glycan receptor specificities of viral surface hemagglutinin from the highly pathogenic Viatnamese H5N1 strains.Introduction of mutations to the avian strains to study path of H5N1 into human population..Hemagglutinin (HA)-antibody pre-complexes were prepared by mixing HA (1mg/ml, 15 <font face="symbol">m</font>l), mouse anti-penta-His-Alexa Fluor488 (0.2mg/m, 35<font face="symbol">m</font>l, Qiagen, Valencia, CA) and anti-mouse-IgG-Alexa Fluor488 (2.3<font face="symbol">m</font>l, 2mg/ml) in a molar ratio of 4:2:1, respectively. Mixtures were incubated for 15 min on ice, diluted with PBS buffer containing 3 percent (w/v) bovine serum albumin (50<font face="symbol">m</font>l) and incubated on the glycan array slide under a microscope cover-glass in a humidified chamber for 30 min. Slides were subsequently washed by successive rinses in PBS with 0.05 percent Tween-20, PBS, and deionized water, then immediately subjected to imaging analysis..Storage: 4 degrees C
Annotation
:
Information not entered/not applicable.
Protein Sample Analyzed
:
Influenza virus A/Duck/Alberta/35/1976 (H1N1) Gly225Asp mutant