Glycan Array Experiment: primscreen_PA_v2_432_08102006
Glycan Array ID
:
primscreen_PA_v2_432_08102006
Sample Name
:
HA5-M2
Sample Category
:
Other
Investigator:
:
Hudson H. Freeze
Download Data:
Sample Information:
Name
:
HA5-M2
Complete Name
:
Hemagglutinin mutant 2
Family
:
Other
Sub Family
:
Influenza A Hemagglutinin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Avian
Estimated Purity
:
Information not entered/not applicable.
Mutation/Chimera
:
A N-linked site was knocked out - Mutation 2
Entry Date [yyyy-mm-dd]
:
2006-09-12
Status
:
Public
Primary Sequence
:
Information not entered/not applicable.
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Avian
External References :
Swissprot
:
Information not entered/not applicable.
Genbank
:
AY818135
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Carbohydrate-binding specificities of extracellular fragments of 3 influenza H5 variants
Associated Resource Requests:
:
cfg_rRequest_600
Glycan Array Version:
:
PA_v2
Protocol Used:
:
Printed Array Screening
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2006-07-19
Supplying Investigator
:
Hudson H. Freeze
Specific Assay Information
:
Sample was incubated onto the array at a concentration of 150 micrograms/ml in PBS with 3 percent BSA and .05 percent Tween 20 for 30 minutes at room temperature in a humidified chamber. A secondary incubation was then performed with anti-penta His Alexa 488 at 50 micrograms/ml for 30 minutes at room temperature in a humidified chamber. ..Storage: -20 C
Annotation
:
1
Protein Sample Analyzed
:
HA5-M2