Glycan Array Experiment: primscreen_910
Glycan Array ID
:
primscreen_910
Sample Name
:
Siglec-6
Sample Category
:
I Type Lectins
Investigator:
:
Bruce S. Bochner
Download Data:
Sample Information:
Name
:
Siglec-6
Complete Name
:
Sialic acid binding Ig-like lectin 6 [Precursor], Siglec-6, Obesity-binding protein 1, OB-BP1, CD33 antigen-like 1
Family
:
Siglec
Sub Family
:
Siglec
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Human
Species Scientific Name
:
Homo sapiens
Estimated Purity
:
Information not entered/not applicable.
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2004-05-24
Status
:
Public
Primary Sequence
:
MLPLLLPLLWAGALAQERRFQLEGPESLTVQEGLCVLVPCRLPTTLPASYYGYGYWFLEGADVPVATNDPDEEVQEETRGRFHLLWDPRRKNCSLSIRDARRRDNAAYFFRLKSKWMKYGYTSSKLSVRVMALTHRPNISIPGTLESGHPSNLTCSVPWVCEQGTPPIFSWMSAAPTSLGPRTTQSSVLTITPRPQDHSTNLTCQVTFPGAGVTMERTIQLNVSYAPQKVAISIFQGNSAAFKILQNTSSLPVLEGQALRLLCDADGNPPAHLSWFQGFPALNATPISNTGVLELPQVGSAEEGDFTCRAQHPLGSLQISLSLFVHWKPEGRAGGVLGAVWGASITTLVFLCVCFIFRVKTRRKKAAQPVQNTDDVNPVMVSGSRGHQHQFQTGIVSDHPAEAGPISEDEQELHYAVLHFHKVQPQEPKVTDTEYSEIKIHK
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
placenta ;placenta_normal ;pooled ;pooled brain, lung, testis
External References :
Genbank
:
D86358
Omim_Parse_Links
:
604405
Swissprot
:
O43699
Omim
:
604405
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Request is for glycan array slides to test freshly isolated transfected and normal human leukocyte subtypes expressing various forms of Siglec-8.
Associated Resource Requests:
:
cfg_rRequest_413
Glycan Array Version:
:
PA_v2
Protocol Used:
:
Printed Array Screening
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2006-06-22
Supplying Investigator
:
Bruce S. Bochner
Specific Assay Information
:
Human Siglec 6 (RhSiglec-6/Fc Chimera from R&D Systems, catalog # 2859-SL, Lot # NXK015061) was assayed in standard binding buffer (TSM, 1 percent BSA, 0.05 percent Tween 20) at a concentration of 200 ug/ml. The slide (70 microliters under a cover slip) was incubated for one hour in a dark humidified chamber. A secondary incubation was performed to with anti-Human IgG AlexaFlour 488 at 5 micrograms/ml, diluted in standard binding buffer and incubated for one hour in a dark humidified chamber.
Annotation
:
not Siglec-8
Protein Sample Analyzed
:
Siglec-6