Glycan Array Experiment: primscreen_1655
Glycan Array ID
:
primscreen_1655
Sample Name
:
E-selectin
Sample Category
:
C Type Lectins
Investigator:
:
Richard D. Cummings
Download Data:
Sample Information:
Name
:
E-selectin
Complete Name
:
Endothelial leukocyte adhesion molecule 1, ELAM-1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2, CD62E antigen
Family
:
C-Type Lectin
Sub Family
:
4-Selectin
Gene Symbol
:
SELE
Synonyms
:
Leukocyte endothelial cell adhesion molecule 2
Species Common Name:
:
Human
Species Scientific Name
:
Homo sapiens
Estimated Purity
:
Information not entered/not applicable.
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2004-05-21
Status
:
Public
Primary Sequence
:
MIASQFLSALTLVLLIKESGAWSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTALSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWDNEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHGSTQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFACPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIPLVAGLSAAGLSLLTLAPFLLWLRKCLRKAKKFVPASSCQSLESDGSYQKPSYIL
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Hypothalamus ;human skeletal muscle ;Chondrosarcoma ;tonsil ;corresponding non cancerous liver tissue ;uterus ;carcinoid ;breast ;endothelial ;leiomyosarcoma ;parathyroid tumor ;heart ;umbilical vein
External References :
Genbank
:
AL021940
Omim_Parse_Links
:
131210
Pdb
:
1ESL
Pdb
:
1G1T
Pdb
:
1KJA
Swissprot
:
P16581
Omim
:
131210
Experiment Information:
Status:
:
Public
Title/Project Description:
:
To determine the fine specificity of P-Selectin-Ig chimera (mouse and human), L-Selectin-Ig chimera (mouse and human), E-Selectin-Ig Chimera (mouse and human).
Associated Resource Requests:
:
cfg_rRequest_979
Glycan Array Version:
:
PA_v3
Protocol Used:
:
Printed Array Screening
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2007-08-29
Supplying Investigator
:
Richard D. Cummings
Specific Assay Information
:
Human E-Selectin was assayed on the slide array at 2ug/ml. The dilution was made in our standard binding buffer (TSM, 1 percent BSA and 0.05 percent Tween 20). 70 microlitres was incubated on the array under a coverslip in a dark humidified chamber for one hour. A secondary incubation was performed with anti-Human IgG Alexa 488 at 5 ug/ml under the same conditions as previously described.
Annotation
:
Human E-Selectin (2ug/ml)
Protein Sample Analyzed
:
E-selectin