Glycan Array Experiment: primscreen_1724
Glycan Array ID
:
primscreen_1724
Sample Name
:
Galectin-3
Sample Category
:
S Type Lectins
Investigator:
:
Richard D. Cummings
Download Data:
Sample Information:
Name
:
Galectin-3
Complete Name
:
Galectin 3, Gal-3, Galactose-specific lectin 3, Mac-2 antigen, IgE-binding protein, 35 kDa lectin, Carbohydrate binding protein 35, CBP 35, Laminin-binding protein, Lectin L-29, L-31, Galactoside-binding protein, GALBP
Family
:
Galectin
Sub Family
:
Galectin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Human
Species Scientific Name
:
Homo sapiens
Estimated Purity
:
Information not entered/not applicable.
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2003-12-10
Status
:
Public
Primary Sequence
:
MADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYHGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSAPGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
melanotic melanoma ;colon ;Pooled human melanocyte, fetal heart, and pregnant uterus ;colonic mucosa from 3 patients with Crohn's disease ;large cell carcinoma ;tumor, 5 pooled (see description) ;well-differentiated endometrial adenocarcinoma, 7 pooled tumors ;hepatic adenoma ;ovary ;2 pooled high-grade transitional cell tumors ;Cervix ;lung_tumor ;three pooled meningiomas ;thymus, pooled ;cervical carcinoma cell line ;senescent fibroblast ;adenocarcinoma ;choriocarcinoma ;hypernephroma ;prostate gland ;glioblastoma with EGFR amplification ;primitive neuroectoderm ;Liver and Spleen ;Adrenal gland ;epithelioid carcinoma ;lung_normal ;prostate ;parathyroid tumor ;colon tumor ;melanotic melanoma, high MDR (cell line) ;malignant melanoma, metastatic to lymph node ;glioblastoma (pooled) ;moderately-differentiated adenocarcinoma ;serous papillary carcinoma, high grade, 2 pooled tumors ;renal cell tumor ;anaplastic oligodendroglioma ;large cell carcinoma, undifferentiated ;melanotic melanoma,
External References :
Genbank
:
BC001120
Pdb
:
1A3K
Pdb
:
1KJL
Pdb
:
1KJR
Omim_Parse_Links
:
153619
Swissprot
:
P17931
Omim
:
153619
Experiment Information:
Status:
:
Public
Title/Project Description:
:
To study the fine specificity of each galectin for high affinity ligands
Associated Resource Requests:
:
cfg_rRequest_977
Glycan Array Version:
:
PA_v3
Protocol Used:
:
Printed Array Screening
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2007-08-16
Supplying Investigator
:
Richard D. Cummings
Specific Assay Information
:
Biotinylated Galectin-3 was assayed on the slide array at 56.5 ug/ml. The dilution was made in our standard binding buffer (TSM, 1 percent BSA and 0.05 percent Tween 20,14mm BME). 70 microlitres was incubated on the array under a coverslip in a dark humidified chamber for one hour. A secondary incubation was performed with Cyanine 5 Steptavidin at 5 ug/ml under the same conditions as previously described.
Annotation
:
(56.5 ug/ml)
Protein Sample Analyzed
:
Galectin-3