Glycan Array Experiment: primscreen_1979
Glycan Array ID
:
primscreen_1979
Sample Name
:
BabA 235 FAdhesin binding fucosylated histo-blood group antigen,
Sample Category
:
Other
Investigator:
:
Lars-Oliver Essen
Download Data:
Sample Information:
Name
:
BabA 235 FAdhesin binding fucosylated histo-blood group antigen,
Complete Name
:
Omp28
Family
:
Other
Sub Family
:
Adhesin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Helicobacter pylori
Estimated Purity
:
Information not entered/not applicable.
Mutation/Chimera
:
Sugar binding domain only
Entry Date [yyyy-mm-dd]
:
2008-04-17
Status
:
Public
Primary Sequence
:
AEDDGFYMSAGYQIGEAAQMVKNTKGIQELSDNYENLSNLLTRYSTLNTLIKLSADPSAVNAARENLGASAKNLIGDTKNSPAYQAVLLAINAAVGFWNVVGYVTQCGGNANGQKSTSSTTIFNNEPGYRSTSITCSLNGHMPGYYGPMSIENFKKLNEAYQILQTALKNGLPALKENNGTVNVTYSYTCSGEGNDNCSLQTTGVTNQNNGTKTETQTIDGKSVTTTISSKVVDSQ
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Expressed in E. coli in inclusion bodies
External References :
Swissprot
:
O52269_HELPY
Genbank
:
HPAG1_0876
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Analysis to identify suitable ligands for the crystallization and structural determination of the Flo5 and Flo11 proteins and BabA adhesion
Associated Resource Requests:
:
cfg_rRequest_1224
Glycan Array Version:
:
PA_v31
Protocol Used:
:
Printed Array Screening
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2008-02-26
Supplying Investigator
:
Lars-Oliver Essen
Specific Assay Information
:
BabA adhesin at pH 2.5 was diluted to 200 micrograms/mL in buffer (10mM KPO4, 200mM NaCl, 2mM MgCl2, 2mM CaCl2). Seventy microliters was applied to the printed surface of the array, coverslipped, and incubated at room temperature in a humidified chamber away from light for two hours.
The coverslip was removed and the slide rinsed 4 times in TSM washing buffer containing Tween and 4 times in TSM buffer without Tween. Seventy microliters of anti-His antibody labeled with Alexa488 at 5ug/ml (Qiagen) was added to the slide for one hour at room temperature in a humidified chamber away from light followed by wash steps.
Annotation
:
Adhesin binding fucosylated histo-blood group antigen assayed at pH 2.5
Protein Sample Analyzed
:
BabA 235 FAdhesin binding fucosylated histo-blood group antigen,