Glycan Array Experiment: primscreen_2054

Glycan Array ID : primscreen_2054
Sample Name : Influenza A virus A/PR/8/34 (H1N1)
Sample Category : Other
Investigator: : Jonathan Yewdell
Download Data:
File: KV7_6821_v3.1_DATA.xls
Conclusive
Sample Information:
Name : Influenza A virus A/PR/8/34 (H1N1)
Complete Name : Information not entered/not applicable.
Family : Other
Sub Family : Viral Hemagglutinin
Gene Symbol : Information not entered/not applicable.
Synonyms : Information not entered/not applicable.
Species Common Name: : Information not entered/not applicable.
Species Scientific Name : Orthomyxoviridae, Influenzavirus A
Estimated Purity : Information not entered/not applicable.
Mutation/Chimera : Information not entered/not applicable.
Entry Date [yyyy-mm-dd] : 2008-01-28
Status : Public
Primary Sequence :
MKANLLVLLCALAAADADTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWLLGNPECDPLLPVRSWSYIVETPNSENGICYPGDFIDYEELREQLSSVSSFERFEIFPKESSWPNHNTNGVTAACSHEGKSSFYRNLLWLTEKEGSYPKLKNSYVNKKGKEVLVLWGIHHPPNSKEQQNLYQNENAYVSVVTSNYNRRFTPEIAERPKVRDQAGRMNYYWTLLKPGDTIIFEANGNLIAPMYAFALSRGFGSGIITSNASMHECNTKCQTPLGAINSSLPYQNIHPVTIGECPKYVRSAKLRMVTGLRNNPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNTVIEKMNIQFTAVGKEFNKLEKRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCDNECMESVRNGTYDYPKYSEESKLNREKVDGVKLESMGIYQILAIYSTVASSLVLLVSLGAISFWMCSNGSLQCRICI
Comments :
Information not entered/not applicable.
Known Sites of Expression :
Information not entered/not applicable.
External References :
Swissprot : AB021709.1
Genbank : EF467821
Experiment Information:
Status: : Public
Title/Project Description: : Continued studies on characterization of the glycan binding properties of the H1N1 mutant viruses
Associated Resource Requests: : cfg_rRequest_1304
Glycan Array Version: : PA_v31
Protocol Used: : Printed Array Screening
Replicate Number: : 1
Result Nature: : Data
Protein/Sample Concentration: : Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]: : 2008-04-21
Supplying Investigator : Jonathan Yewdell
Specific Assay Information : Sample was assayed on the slide array at a concentration of 5000 HAU/ml. The dilution was made in our standard binding buffer (TSM, 1 percent BSA and 0.05 percent Tween 20). 70 microlitres was incubated on the array under a coverslip in a dark humidified chamber for one hour at room temperature. Secondary incubation was done with Alexa488 labeled NA2-1C1 antibody at 5 ug/ml. Samples were stored at -80C.
Annotation : KV7: K165E+N231K mutations in HA
Protein Sample Analyzed : Influenza A virus A/PR/8/34 (H1N1)