Glycan Array Experiment: primscreen_2160
Glycan Array ID
:
primscreen_2160
Sample Name
:
Langerin ectodomain (P830)
Sample Category
:
C Type Lectins
Investigator:
:
Gerard Zurawski
Download Data:
Sample Information:
Name
:
Langerin ectodomain (P830)
Complete Name
:
Langerin, CLEC4K
Family
:
C-Type Lectin
Sub Family
:
C-type Lectin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Homo Sapiens
Estimated Purity
:
Information not entered/not applicable.
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2007-12-19
Status
:
Public
Primary Sequence
:
LDYPRFMGTISDVKTNVQLLKGRVDNISTLDSEIKKNSDGMEAAGVQIQMVNESLGYVRSQFLKLKTSVEKANAQIQILTRSWEEVSTLNAQIPELKSDLEKASALNTKIRALQGSLENMSKLLKRQNDILQVVSQGWKYFKGNFYYFSLIPKTWYSAEQFCVSRNSHLTSVTSESEQEFLYKTAGGLIYWIGLTKAGMEGDWSWVDDTPFNKVQSARFWIPGEPNNAGNNEHCGNIKAPSLQAWNDAPCDKTFLFICKRPYVPSEPVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSR
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Transiently transfected 293F cells
External References :
Swissprot
:
CLC4K_HUMAN
Genbank
:
AJ242859.1 GI:6523278|
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Glycoarray analysis of ectodomain IgGFc fusion proteins 334998 and 335774 to define which glycans have the highest affinity
Associated Resource Requests:
:
cfg_rRequest_1244
Glycan Array Version:
:
PA_v31
Protocol Used:
:
Printed Array Screening
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
10 μg/ml
Experiment Date [yyyy-mm-dd]:
:
2008-03-21
Supplying Investigator
:
Gerard Zurawski
Specific Assay Information
:
Langerin was diluted to 10 micrograms/mL in PBS binding buffer (PBS, 1 percent BSA and 0.05 percent Tween 20). Seventy microliters was applied to the printed surface of the array, coverslipped, and incubated at room temperature in a humidified chamber away from light for one hour.
The coverslip was removed and the slide rinsed 4 times in PBS washing buffer containing Tween and 4 times in PBS buffer without Tween. Seventy microliters of PE-labeled goat anti-human Fc gamma (5 ug/ml, provided by PI) in PBS binding buffer was added to the slide for one hour at room temperature in a humidified chamber away from light. Washes were performed as above.
Annotation
:
Langerin 10ug/ml
Protein Sample Analyzed
:
Langerin ectodomain (P830)