Glycan Array Experiment: primscreen_2932
Glycan Array ID
:
primscreen_2932
Sample Name
:
Influenza virus A/New_York/1/1918 (H1N1) Asp190Glu mutant
Sample Category
:
Other
Investigator:
:
CoreD CFG
Download Data:
Could not find file.
Sample Information:
Name
:
Influenza virus A/New_York/1/1918 (H1N1) Asp190Glu mutant
Complete Name
:
Influenza virus A/New_York/1/1918 (H1N1) Hemagglutinin Asp190Glu mutant
Family
:
Other
Sub Family
:
Viral lectin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Influenza A virus (A/New_York/1/1918 (H1N1))
Estimated Purity
:
His Tag fusion
Mutation/Chimera
:
Asp190Glu
Entry Date [yyyy-mm-dd]
:
2006-03-10
Status
:
Public
Primary Sequence
:
MEARLLVLLCAFAATNADTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCKLKGIAPLQLGKCNIAGWLLGNPECDLLLTASSWSYIVETSNSENGTCYPGDFIDYEELREQLSSVSSFEKFEIFPKTSSWPNHETTKGVTAACSYAGASSFYRNLLWLTKKGSSYPKLSKSYVNNKGKEVLVLWGVHHPPTGTEQQSLYQNADAYVSVGSSKYNRRFTPEIAARPKVRGQAGRMNYYWTLLEPGDTITFEATGNLIAPWYAFALNRGSGSGIITSDAPVHDCNTKCQTPHGAINSSLPFQNIHPVTIGECPKYVRSTKLRMATGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAIDGITNKVNSVIEKMNTQ
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Information not entered/not applicable.
External References :
Swissprot
:
Information not entered/not applicable.
Genbank
:
Information not entered/not applicable.
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Information not entered/not applicable.
Associated Resource Requests:
:
cfg_rRequest_D2
Glycan Array Version:
:
PA_v1
Protocol Used:
:
Printed Array Screening
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
15 μg/ml
Experiment Date [yyyy-mm-dd]:
:
2005-12-05
Supplying Investigator
:
CoreD CFG
Specific Assay Information
:
Glycan receptor specificities of viral surface hemagglutinin glycoproteins in H1 and H3 avian and human Influenza viruses..Hemagglutinin (HA)-antibody pre-complexes were prepared by mixing HA (1mg/ml, 15 ml), mouse anti-penta-His-Alexa Fluor488 (0.2mg/m, 35ml, Qiagen, Valencia, CA) and anti-mouse-IgG-Alexa Fluor488 (2.3ml, 2mg/ml) in a molar ratio of 4:2:1, respectively. Mixtures were incubated for 15 min on ice, diluted with PBS buffer containing 3 percent (w/v) bovine serum albumin (50ml) and incubated on the glycan array slide under a microscope cover-glass in a humidified chamber for 30 min. Slides were subsequently washed by successive rinses in PBS with 0.05 percent Tween-20, PBS, and deionized water, then immediately subjected to imaging analysis..Storage: 4 degrees C
Annotation
:
Influenza virus A/New_York/1/1918 (H1N1) Asp190Glu mutant
Protein Sample Analyzed
:
Influenza virus A/New_York/1/1918 (H1N1) Asp190Glu mutant