Glycan Array Experiment: primscreen_3196
Glycan Array ID
:
primscreen_3196
Sample Name
:
H1N1 hemagglutinin- Kentucky
Sample Category
:
Other
Investigator:
:
Peter P.J.M Rottier
Download Data:
Sample Information:
Name
:
H1N1 hemagglutinin- Kentucky
Complete Name
:
Hemagglutinin serotype 1
Family
:
Viral / Bacterial Lectin
Sub Family
:
Type 1 viral envelope protein
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Influenza A virus (A/Kentucky/UR06-0258/2007/H1N1)
Estimated Purity
:
95%
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2010-05-17
Status
:
Private
Primary Sequence
:
MPMGSLQPLATLYLLGMLVASVLADTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCLLKGIAPLQLGNCSVAGWILGNPECELLISKESWSYIVETPNPENGTCYPGYFADYEELREQLSSVSSFERFEIFPKESSWPNHTVTGVSASCSHNGRSSFYRNLLWLTGKNGLYPSLSKSYANNKEKEVLVLWGVHHPPNIGDQRALYHTENAYVSVVSSHYSRRFTPEIAKRPKVRDQEGRINYYWTLLEPGDTIIFEANGNLIAPRFAFALSRGFGSGIITSNAPMDECDAKCQTPQGAINSSLPFQNVHPVTIGECPKYVRSTKLRMVTGLRNIPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNSVIEKMNTQFTAVGKEFNKLERRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCNDECMESVKNGTYDYPKYSEESKLNREKIDGVKLELIKRMKQIEDKIEEIESKQKKIENEIARIKKIKLVPRGSLEWSHPQFEK
Comments
:
Fetuin Solid Phase, Hemagglutination.
Known Sites of Expression
:
293T GnT1-
External References :
Swissprot
:
Information not entered/not applicable.
Genbank
:
ABX58635.1
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Continued studies of RR#1511, RR#1611, RR#1671, RR#1797 - to develop a binding profile for these additional hemagglutinin (HA) proteins derived from different serotypes of avian influenza A viruses.
Associated Resource Requests:
:
cfg_rRequest_1882
Glycan Array Version:
:
PA_v41
Protocol Used:
:
Protocol Tertiary step
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2010-02-02
Supplying Investigator
:
Peter P.J.M Rottier
Specific Assay Information
:
Samples were precomplexed with antibody: 24 ul Binding Buffer in a tube, added 32.5 ul sample and mixed, added 9 ul of anti-Strep mAb (sent by PI) and 4.5 ul Alexa488-labeled anti-mouse IgG (Invitrogen), mixed well, and incubated on ice for 20 minutes. The 13 ug HA precomplex was added to the slide for 2 hours at room temp.
Annotation
:
Hemagglutinin serotpye 1 from HEK 293T GnT1- cells (H1N1- Kentucky)
Protein Sample Analyzed
:
H1N1 hemagglutinin- Kentucky