Glycan Array Experiment: primscreen_3336
Glycan Array ID
:
primscreen_3336
Sample Name
:
A/Swine/Ohio/891/01 (H1N2)
Sample Category
:
Other
Investigator:
:
Peter P.J.M Rottier
Download Data:
Sample Information:
Name
:
A/Swine/Ohio/891/01 (H1N2)
Complete Name
:
Hemagglutinin serotype 5
Family
:
Viral / Bacterial Lectin
Sub Family
:
Type 1 viral envelope protein
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
[Influenza A virus (A/Swine/Ohio/891/01
Estimated Purity
:
95%
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2010-08-11
Status
:
Private
Primary Sequence
:
MKAILVVLLYTFTTANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDRHNGKLCKLRGVAPLHLGKCNIAGWLLGNPECESLFTASSWSYIVETSNSDNGTCYPGDFINYEELREQLSSVSSFERFEIFPKTSSWPNHDTNRGVTAACPHAGAKSFYRNLIWLVKKGNSYPKISKSYINNKEKEVLVLWGIHHPSTSADQQSLYQNADAYVFVGSSRYSKKFKPEIATRPKVRDQAGRMNYYWTLVEPGDKITFEATGNLVVPRYAFALKRNSGSGIIISDTSVHDCNTTCQTPKGAINTSLPFQNIHPVTIGECPKYVKSTKLRMATGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADLKSTQNAIDGITNKVNSVIEKMNTQFTAVGKEFSHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDDTCMESVKNGTYDYPKYSEEAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAISFWMCSNGSLQCRICI
Comments
:
Fetuin Solid Phase, Hemagglutination
Known Sites of Expression
:
293T GnT1-, PUBMED 11880444
External References :
Swissprot
:
Information not entered/not applicable.
Genbank
:
AAL87865.1
Experiment Information:
Status:
:
Public
Title/Project Description:
:
continued studies RR#1882 - to determine glycan binding specificity of 5 influenza A hemagglutinin (HA) samples
Associated Resource Requests:
:
cfg_rRequest_1981
Glycan Array Version:
:
PA_v41
Protocol Used:
:
Protocol Direct Binding
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2010-03-12
Supplying Investigator
:
Peter P.J.M Rottier
Specific Assay Information
:
Samples were precomplexed with antibody: 46 ul Binding Buffer in a tube, added 17.5 ul sample and mixed, added 4.5 ul of anti-Strep mAb (sent by PI) and 2.25 ul Alexa488-labeled anti-mouse IgG (Invitrogen), mixed well, and incubated on ice for 20 minutes. The 7 ug HA precomplex was added to the slide for 2 hours at room temp.
Annotation
:
Swine/Ohio/891/01 H1N2
Protein Sample Analyzed
:
A/Swine/Ohio/891/01 (H1N2)