Glycan Array Experiment: primscreen_3337

Glycan Array ID : primscreen_3337
Sample Name : H1N1 hemagglutinin- Kentucky
Sample Category : Other
Investigator: : Peter P.J.M Rottier
Download Data:
File: kentucky_2007_11631_v4.1_DATA.xls
Conclusive
Sample Information:
Name : H1N1 hemagglutinin- Kentucky
Complete Name : Hemagglutinin serotype 1
Family : Viral / Bacterial Lectin
Sub Family : Type 1 viral envelope protein
Gene Symbol : Information not entered/not applicable.
Synonyms : Information not entered/not applicable.
Species Common Name: : Information not entered/not applicable.
Species Scientific Name : Influenza A virus (A/Kentucky/UR06-0258/2007/H1N1)
Estimated Purity : 95%
Mutation/Chimera : Information not entered/not applicable.
Entry Date [yyyy-mm-dd] : 2010-05-17
Status : Private
Primary Sequence :
MPMGSLQPLATLYLLGMLVASVLADTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCLLKGIAPLQLGNCSVAGWILGNPECELLISKESWSYIVETPNPENGTCYPGYFADYEELREQLSSVSSFERFEIFPKESSWPNHTVTGVSASCSHNGRSSFYRNLLWLTGKNGLYPSLSKSYANNKEKEVLVLWGVHHPPNIGDQRALYHTENAYVSVVSSHYSRRFTPEIAKRPKVRDQEGRINYYWTLLEPGDTIIFEANGNLIAPRFAFALSRGFGSGIITSNAPMDECDAKCQTPQGAINSSLPFQNVHPVTIGECPKYVRSTKLRMVTGLRNIPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNSVIEKMNTQFTAVGKEFNKLERRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCNDECMESVKNGTYDYPKYSEESKLNREKIDGVKLELIKRMKQIEDKIEEIESKQKKIENEIARIKKIKLVPRGSLEWSHPQFEK
Comments :
Fetuin Solid Phase, Hemagglutination.
Known Sites of Expression :
293T GnT1-
External References :
Swissprot : Information not entered/not applicable.
Genbank : ABX58635.1
Experiment Information:
Status: : Public
Title/Project Description: : continued studies RR#1882 - to determine glycan binding specificity of 5 influenza A hemagglutinin (HA) samples
Associated Resource Requests: : cfg_rRequest_1981
Glycan Array Version: : PA_v41
Protocol Used: : Protocol Direct Binding
Replicate Number: : 1
Result Nature: : Data
Protein/Sample Concentration: : Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]: : 2010-03-10
Supplying Investigator : Peter P.J.M Rottier
Specific Assay Information : Samples were precomplexed with antibody: 46 ul Binding Buffer in a tube, added 17.5 ul sample and mixed, added 4.5 ul of anti-Strep mAb (sent by PI) and 2.25 ul Alexa488-labeled anti-mouse IgG (Invitrogen), mixed well, and incubated on ice for 20 minutes. The 7 ug HA precomplex was added to the slide for 2 hours at room temp.
Annotation : A/Kentucky H1N1
Protein Sample Analyzed : H1N1 hemagglutinin- Kentucky