Glycan Array Experiment: primscreen_3474
Glycan Array ID
:
primscreen_3474
Sample Name
:
Influenza A hemagglutinin H1N1
Sample Category
:
Other
Investigator:
:
Peter P.J.M Rottier
Download Data:
Sample Information:
Name
:
Influenza A hemagglutinin H1N1
Complete Name
:
Information not entered/not applicable.
Family
:
Other
Sub Family
:
Influenza virus hemaggluinin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Homo sapiens
Estimated Purity
:
Information not entered/not applicable.
Mutation/Chimera
:
Deletion of TMD and cytoplasmic tail, added trimerization fold, linker, His tag, Strep tag
Entry Date [yyyy-mm-dd]
:
2009-09-09
Status
:
Private
Primary Sequence
:
adlgsadpgdtlcigyhannstdtvdtvleknvtvthsvnlledkhngklcklrgvaplhlgkcniagwilgnpeceslstasswsyivetpssdngtcypgdfidyeelreqlssvssferfeifpktsswpnhdsnkgvtaacphagaksfyknliwlvkkgnsypklsksyindkgkevlvlwgihhpstsadqqslyqnadtyvfvgssryskkfkpeiairpkvrdqegrmnyywtlvepgdkitfeatgnlvvpryafamernagsgiiisdtpvhdcnttcqtpkgaintslpfqnihpitigkcpkyvkstklrlatglrnipsiqsrglfgaiagfieggwtgmvdgwygyhhqneqgsgyaadlkstqnaideitnkvnsviekmntqftavgkefnhlekrienlnkkvddgfldiwtynaellvllenertldyhdsnvknlyekvrsqlknnakeigngcfefyhkcdntcmesvkngtydypkyseeaklnreeidgvrslvprgspgsgyipeaprdgqayvrkdgewvllstflghhhhhhggswshpqfek
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Expressed in baculovirus system
External References :
Swissprot
:
C3W5S1
Genbank
:
ACP41105
Experiment Information:
Status:
:
Public
Title/Project Description:
:
Continued studies RR#1611, #1671, #1797, and #1882 - to identify the critical HA residues involved in receptor recognition
Associated Resource Requests:
:
cfg_rRequest_2066
Glycan Array Version:
:
PA_v41
Protocol Used:
:
Protocol Direct Binding
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2010-07-26
Supplying Investigator
:
Peter P.J.M Rottier
Specific Assay Information
:
Sample was precomplexed with antibody: 45.8 ul Binding Buffer in a tube, added 17.5 ul sample and mixed, added 4.5 ul of anti-Strep mAb (sent by PI) and 2.25 ul Alexa488-labeled anti-mouse IgG (Invitrogen), mixed well, and incubated on ice for 20 minutes. The 7 ug HA precomplex was added to the slide for 2 hours at room temp.
Annotation
:
Influenza A virus hemagglutinin (A/California/04/2009) H1N1, E223A mutant HA
Protein Sample Analyzed
:
Influenza A hemagglutinin H1N1