Glycan Array Experiment: primscreen_3475

Glycan Array ID : primscreen_3475
Sample Name : Influenza A viral hemagglutinin (A/Puerto Rico/8/34) H1N1
Sample Category : Other
Investigator: : Peter P.J.M Rottier
Download Data:
File: PR8_12896_v4.1_DATA.xls
Conclusive
Sample Information:
Name : Influenza A viral hemagglutinin (A/Puerto Rico/8/34) H1N1
Complete Name : Hemagglutinin serotype 1
Family : Viral / Bacterial Lectin
Sub Family : Hemagglutinin
Gene Symbol : Information not entered/not applicable.
Synonyms : Information not entered/not applicable.
Species Common Name: : Information not entered/not applicable.
Species Scientific Name : Information not entered/not applicable.
Estimated Purity : 95%
Mutation/Chimera : Information not entered/not applicable.
Entry Date [yyyy-mm-dd] : 2010-12-08
Status : Private
Primary Sequence :
mkanllvllcalaaadadticigyhannstdtvdtvleknvtvthsvnlledshngklcrlkgiaplqlgkcniagwllgnpecdpllpvrswsyivetpnsengicypgdfidyeelreqlssvssferfeifpkesswpnhnttkgvtaacshagkssfyrnllwltekegsypklknsyvnkkgkevlvlwgihhpsnskdqqniyqnenayvsvvtsnynrrftpeiaerpkvrdqagrmnyywtllkpgdtiifeangnliapryafalsrgfgsgiitsnasmhecntkcqtplgainsslpfqnihpvtigecpkyvrsaklrmvtglrnipsiqsrglfgaiagfieggwtgmidgwygyhhqneqgsgyaadqkstqnaingitnkvnsviekmniqftavgkefnklekrmenlnkkvddgfldiwtynaellvllenertldfhdsnvknlyekvksqlknnakeigngcfefyhkcdnecmesvrngtydypkyseesklnrekvdgvklesmgiyqilaiystvasslvllvslgaisfwmcsngslqcrici
Comments :
Fetuin Solid Phase, Hemagglutination
Known Sites of Expression :
293T GnT1-
External References :
Swissprot : Information not entered/not applicable.
Genbank : NP_040980.1
Experiment Information:
Status: : Public
Title/Project Description: : Continued studies RR#1611, #1671, #1797, and #1882 - to identify the critical HA residues involved in receptor recognition
Associated Resource Requests: : cfg_rRequest_2066
Glycan Array Version: : PA_v41
Protocol Used: : Protocol Direct Binding
Replicate Number: : 1
Result Nature: : Data
Protein/Sample Concentration: : Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]: : 2010-06-07
Supplying Investigator : Peter P.J.M Rottier
Specific Assay Information : Sample was precomplexed with antibody: 45.8 ul Binding Buffer in a tube, added 17.5 ul sample and mixed, added 4.5 ul of anti-Strep mAb (sent by PI) and 2.25 ul Alexa488-labeled anti-mouse IgG (Invitrogen), mixed well, and incubated on ice for 20 minutes. The 7 ug HA precomplex was added to the slide for 2 hours at room temp.
Annotation : Influenza A virus hemagglutinin (A/Puerto Rico/8/34) H1N1
Protein Sample Analyzed : Influenza A viral hemagglutinin (A/Puerto Rico/8/34) H1N1