Glycan Array Experiment: primscreen_3779
Glycan Array ID
:
primscreen_3779
Sample Name
:
Burkholderia oklahomensis agglutinin(BOA)
Sample Category
:
Other
Investigator:
:
Angela M. Gronenborn
Download Data:
Sample Information:
Name
:
Burkholderia oklahomensis agglutinin(BOA)
Complete Name
:
Information not entered/not applicable.
Family
:
Viral / Bacterial Lectin
Sub Family
:
bacterial lectin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Burkholderia oklahomensis EO147 proteobacterium
Estimated Purity
:
>95%
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2011-04-10
Status
:
Private
Primary Sequence
:
svdmtktskgfnnlqhvqnqwggssapwheggmwvlgcrsgqnvvalniksgdggrtltgtmtyvgegpigfratltqsntyavenqwggssapwhpggtwvigcrvnqqvvaldiesgdqgatlagtmtyagegpigfksqqadggvyavenqwggssapwhnggvwvigardqavvavsigstdsgktlngnmtyagegpigfkgnsvagnnyavenqwggtsapwhpggiwllgcrsgqnvvelyitsgdngntfhgsmtysgegpigframalpq
Comments
:
Information not entered/not applicable.
Known Sites of Expression
:
Expressed in E. coli BL21 DE3 cells; Fish Sci. (2009) 75: 743-753
External References :
Swissprot
:
Information not entered/not applicable.
Genbank
:
Information not entered/not applicable.
Experiment Information:
Status:
:
Public
Title/Project Description:
:
To investigate binding specificities of the 4-binding-site members of the OAA family (OAA-family lectin from the proteobacterium Burkholderia oklahomensis EO14) for comparison to the rest of the OAA family and other anti-HIV lectins
Associated Resource Requests:
:
cfg_rRequest_2208
Glycan Array Version:
:
PA_v5
Protocol Used:
:
Protocol Direct Binding
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
200 μg/ml
Experiment Date [yyyy-mm-dd]:
:
2011-03-17
Supplying Investigator
:
Angela M. Gronenborn
Specific Assay Information
:
Sample was diluted in 25 mM sodium acetate, 25 mM sodium chloride buffer pH 5.0, 4 mM DTT, 1% BSA and was directly labeled with AF488.
Annotation
:
BOA lectin (MJW)
Protein Sample Analyzed
:
Burkholderia oklahomensis agglutinin(BOA)