Glycan Array Experiment: primscreen_3890
Glycan Array ID
:
primscreen_3890
Sample Name
:
Siglec-7-biotin
Sample Category
:
I Type Lectins
Investigator:
:
Ingo Mueller
Download Data:
Sample Information:
Name
:
Siglec-7-biotin
Complete Name
:
CD328; p75/AIRM1; QA79
Family
:
Siglec
Sub Family
:
Siglec; CD33-related siglec
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Human; Homo sapiens
Estimated Purity
:
SDS-PAGE; (protein purification was performed by FPLC)
Mutation/Chimera
:
the V-Ig-set and two C2-Ig-sets are expressed, additionally a biotinylation sequence was added and the recombinant protein was enzymatically biotinylated after purification
Entry Date [yyyy-mm-dd]
:
2011-12-16
Status
:
Public
Primary Sequence
:
MLLLLLLPLLWGRERVEGQKSNRKDYSLTMQSSVTVQEGMCVHVRCSFSYPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNPAWAVQEETRDRFHLLGDPQTKNCTLSIRDARMSDAGRYFFRMEKGNIKWNYKYDQLSVNVTALTHRPNILIPGTLESGCFQNLTCSVPWACEQGTPPMISWMGTSVSPLHPSTTRSSVLTLIPQPQHHGTSLTCQVTLPGAGVTTNRTIQLNVSYPPQNLTVTVFQGEGTASTALGNSSSLSVLEGQSLRLVCAVDSNPPARLSWTWRSLTLYPSQPSNPLVLELQVHLGDEGEFTCRAQNSLGSQHVSLNLSLQQEYTGKMRPVSGVLLGAVGGAGATALVFLSFCVIFIVVRSCRKKSARPAADVGDIGMKDANTIRGSASQGNLTESWADDNPRHHGLAAHSSGEEREIQYAPLSFHKGEPQDLSGQEATNNEYSEIKIPK
Comments
:
The purified siglec-7 protein was enzymatically biotinylated and then tetramers were produced by binding to streptavidin-PE. The activity was analysed by flow cytometry; active siglec-tetramers bound to cell surface of PBMC.
Known Sites of Expression
:
Recombinant protein, expressed in the stably transfected human cell line 293T
External References :
Swissprot
:
Q9Y286
Genbank
:
NM_014385; AF170485
Experiment Information:
Status:
:
Public
Title/Project Description:
:
To determine the specificity of the tetrameric siglecs on the glycan array compared to the monomeric siglecs.
Associated Resource Requests:
:
cfg_rRequest_2130
Glycan Array Version:
:
PA_v42
Protocol Used:
:
Protocol Direct Binding
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
180 μg/ml
Experiment Date [yyyy-mm-dd]:
:
2011-03-30
Supplying Investigator
:
Ingo Mueller
Specific Assay Information
:
Biotin-labeled sample was precomplexed by PI with phycoerythrine-labeled streptavidin. Sample at 200 ug/ml was diluted with 10% BSA (final concentration 1% BSA) and added to slide for 1hr. at RT.
Annotation
:
Siglec-7-biotin
Protein Sample Analyzed
:
Siglec-7-biotin