Glycan Array Experiment: primscreen_4247
Glycan Array ID
:
primscreen_4247
Sample Name
:
Influenza A viral hemagglutinin (A/Puerto Rico/8/34) H1N1
Sample Category
:
Other
Investigator:
:
Peter P.J.M Rottier
Download Data:
Sample Information:
Name
:
Influenza A viral hemagglutinin (A/Puerto Rico/8/34) H1N1
Complete Name
:
Hemagglutinin serotype 1
Family
:
Viral / Bacterial Lectin
Sub Family
:
Hemagglutinin
Gene Symbol
:
Information not entered/not applicable.
Synonyms
:
Information not entered/not applicable.
Species Common Name:
:
Information not entered/not applicable.
Species Scientific Name
:
Information not entered/not applicable.
Estimated Purity
:
95%
Mutation/Chimera
:
Information not entered/not applicable.
Entry Date [yyyy-mm-dd]
:
2010-12-08
Status
:
Private
Primary Sequence
:
mkanllvllcalaaadadticigyhannstdtvdtvleknvtvthsvnlledshngklcrlkgiaplqlgkcniagwllgnpecdpllpvrswsyivetpnsengicypgdfidyeelreqlssvssferfeifpkesswpnhnttkgvtaacshagkssfyrnllwltekegsypklknsyvnkkgkevlvlwgihhpsnskdqqniyqnenayvsvvtsnynrrftpeiaerpkvrdqagrmnyywtllkpgdtiifeangnliapryafalsrgfgsgiitsnasmhecntkcqtplgainsslpfqnihpvtigecpkyvrsaklrmvtglrnipsiqsrglfgaiagfieggwtgmidgwygyhhqneqgsgyaadqkstqnaingitnkvnsviekmniqftavgkefnklekrmenlnkkvddgfldiwtynaellvllenertldfhdsnvknlyekvksqlknnakeigngcfefyhkcdnecmesvrngtydypkyseesklnrekvdgvklesmgiyqilaiystvasslvllvslgaisfwmcsngslqcrici
Comments
:
Fetuin Solid Phase, Hemagglutination
Known Sites of Expression
:
293T GnT1-
External References :
Swissprot
:
Information not entered/not applicable.
Genbank
:
NP_040980.1
Experiment Information:
Status:
:
Public
Title/Project Description:
:
continued studies RR#2211 - ongoing research on influenza A virus HA-glycan interactions by testing another set of mutant HA proteins for their ability to bind sialylated glycans
Associated Resource Requests:
:
cfg_rRequest_2261
Glycan Array Version:
:
PA_v5
Protocol Used:
:
Protocol Direct Binding
Replicate Number:
:
1
Result Nature:
:
Data
Protein/Sample Concentration:
:
200 μg/ml
Experiment Date [yyyy-mm-dd]:
:
2011-06-02
Supplying Investigator
:
Peter P.J.M Rottier
Specific Assay Information
:
Sample was precomplexed with antibody: 21.5 ul Binding Buffer in a tube, added 35 ul sample and mixed, added 4.5 ul of anti-Strep mAb (sent by PI) and 4.5 ul Alexa488-labeled anti-mouse IgG (Invitrogen), mixed well, and incubated on ice for 20 minutes. The 14 ug HA precomplex was added to the slide for 2 hours at room temp.
Annotation
:
Influenza HA (A/Puerto Rico/8/34), mutant D190E
Protein Sample Analyzed
:
Influenza A viral hemagglutinin (A/Puerto Rico/8/34) H1N1