Protocol ID: Protocol Biotinylated Samples

Title : Protocol Biotinylated Samples
Category : Lectin-Glycan Binding
Overview : Biotinylated sample is diluted to an appropriate concentration in standard binding buffer (TSM, 1% BSA and 0.05% Tween 20). Seventy microliters is applied to the printed surface of the array, cover slip is applied, and incubated at room temperature in a humidified chamber away from light for one hour. The cover slip is removed and the slide is rinsed 4 times in standard TSM washing buffer containing 0.05% Tween and 4 times in TSM buffer without Tween. Seventy microliters of fluorescently labeled streptavidin in standard TSM binding buffer is added to the slide for one hour at room temperature in a humidified chamber away from light as described. Washes are performed as above, followed by 4 washes in distilled water.
Procedure : See attached PDF file
File : Protocol Biotinylated Samples.pdf