Protocol ID: Protocol Direct Binding
Title
:
Protocol Direct Binding
Category
:
Lectin-Glycan Binding
Overview
:
Fluorescently labeled sample is diluted to an appropriate concentration in standard binding buffer (TSM, 1% BSA and 0.05% Tween 20). Seventy microliters is applied to the printed surface of the array, cover slip is applied, and incubated at room temperature in a humidified chamber away from light for one hour. The cover slip is removed and the slide is rinsed 4 times in standard TSM washing buffer containing 0.05% Tween, 4 times in TSM buffer without Tween, and 4 times in distilled water.
Procedure
:
See attached PDF file