Request ID: cfg_rRequest_1118
Status
:
Approved
Project Description
:
50 glycan arrays for the study of the specificities of human, avian and swine influenza viruses
CFG Member
:
Other
Requester First Name
:
James
Requester Last Name
:
Paulson
Head of Lab First Name
:
James
Head of Lab Last Name
:
Paulson
Assigned First Name
:
James
Assigned Last Name
:
Paulson
Requester Email
:
jpaulson@scripps.edu
Requester Interest
:
Dr. Paulson's group investigates the roles of carbohydrate-binding proteins involved in immune regulation and human disease, through their interaction with carbohydrate groups expressed on cell surface glycoconjugates. This laboratory is currently focused on how sialoside ligands modulate Siglec functions and on understanding the molecular basis of glycosylation changes following differentiation and activation of leukocytes.
Request Date [yyyy-mm-dd]
:
2007-08-08
Institution
:
The Scripps Research Institute
Shipping Address
:
Department of Chemical Physiology, MEM-L71
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla, CA 92037
858-784-9634
Comments
:
Information not entered/not applicable.
CFG Core
:
D
Resource Type
:
Carbohydrate
Amount Requested
:
50 Glycan Arrays
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2026-04-13
Experiment to be conducted
:
This request for 50 glycan arrays is a continuation of resource request 451 covering collaborative projects of the Paulson lab with the laboratories of Ian Wilson (TSRI), and of Robert Webster and Richard Webby (St. Judes Childrens Hospital) on the specificities of human, avian and swine influenza viruses. The previous request of 45 glycan arrays has resulted in three publications with the Wilson lab on the elucidation of the amino acids responsible for conversion of the (2-3) receptor specificities of the H5 hemagglutinin to (2-6) type specificity of avian viruses. In the collaboration with Robert Webster and Richard Webby we tested various model viruses with and without inactivating agents to determine how pathogenic strains might be handled for analysis. We established that BPL (beta-propiolactone) inactivation of intact influenza viruses did not alter receptor specificity, allowing us to receive BPL inactivated avian viruses. The collaboration with the Webster group is now focusing on survey of field isolates from certain avian species (e.g. quail) and swine deemed significant with respect to the evolution of the avian flu and its potential to cross the avian to human species barrier. Series of H6, H9 and H2 viruses across avian and swine species will be examined, with the first 20 strains receivd and waiting to be assessed. It is proposed that the virus adsorption and data analysis be done by members of the Paulson group, and data be provided to Core D for uploading into the CFG database under the direction of Core D, as done for resource request 451.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Ryan McBride
GBP being Addressed
:
Influenza virus hemagglutinin
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands.