Request ID: cfg_rRequest_1351
Status
:
Approved
Project Description
:
To continued the studies of human collectin CL-K1 see cfg_rRequest_1194.
CFG Member
:
Not Member
Requester First Name
:
Katsuki
Requester Last Name
:
Ohtani
Head of Lab First Name
:
Nobutaka
Head of Lab Last Name
:
Wakamiya
Assigned First Name
:
Nobutaka
Assigned Last Name
:
Wakamiya
Requester Email
:
ohtani@asahikawa-med.ac.jp
Requester Interest
:
Information not entered/not applicable.
Request Date [yyyy-mm-dd]
:
2008-03-31
Institution
:
Department of Microbiology and Immunochemistry, Asahikawa Medical College
Shipping Address
:
1-1-1 Midorigaoka-Higashi-Nijo, Asahikawa, Hokkaido 078-8510, Japan
Comments
:
Information not entered/not applicable.
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2008-04-07
Experiment to be conducted
:
Our group studies the biological functions of collectins. Recently we found new collectin CL-K1. The deduced amino acid sequence (Glu-Pro-Asn) of sugar frame in CL-K1 indicates binding specificity for mannose, glucose, fucose and GlcNAc, but CL-K1 was not bind with GlcNAc like MBL. Last month we received the results of glycan array screening (refer the Request ID : 1194). These results were very interesting. Then, we newly prepared recombinant CL-K1 and labeled with Alexa 488. Therefore we would like to confirm this result and do additional condition. (1) addition of 5ul of 200mM CaCl2 and 5uL of 200ug/ml PolyI, (2) addition of 5ul of 200mM CaCl2 and 5uL of 200ug/ml PolyC, (3) addition of 5ul of 200mM CaCl2 and 5uL of 200ug/ml Dextran sulfate to 100uL of sample.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Information not entered/not applicable.
GBP being Addressed
:
We will send Alexa488 labeled recombinant human CL-K1 in recommended binding buffer and additional reagents 200ug/mL of PolyI, PolyC and Dextran sulfate solution
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands.