Request ID: cfg_rRequest_2058
Status
:
Approved
Project Description
:
To examine the glycan-specificity of these bacterial lectin, Chromobacterium violaceum, Burkholderia cenocepacia, and Ralstonia solanacearum on the most recent version of the array
CFG Member
:
Member
Requester First Name
:
Anne
Requester Last Name
:
Imberty
Head of Lab First Name
:
Anne
Head of Lab Last Name
:
Imberty
Assigned First Name
:
Anne
Assigned Last Name
:
Imberty
Requester Email
:
imberty@cermav.cnrs.fr
Requester Interest
:
This laboratory focuses on structural and thermodynamical characterization of lectin-carbohydrate interactions.
Request Date [yyyy-mm-dd]
:
2010-04-28
Institution
:
CERMAV-CNRS
Shipping Address
:
Information not entered/not applicable.
Comments
:
Information not entered/not applicable.
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2012-06-04
Experiment to be conducted
:
Soluble lectins from Pseudomonas aeruginosa have been demonstrated to be involved in the adhesion to epithelial cells and in the infection process. PA-IL (LecA - galactose specific) and PA-IIL (LecB - fucose specific) have been extensively studied for their structure and inhibition and PA-IIL has been selected among the paradigms for GBP in the consortium. Nevertheless, their specificity was not tested on the recent version of the glycan array (specially PA-IL that was only tested on the first version of the plate). In collaboration with David Smith we undergo a complete specificity caracterisation of Pseudomonas aeruginosa soluble lectins including comparison with the ones from related opportunistic bacteria Chromobacterium violaceum,Burkholderia cenocepacia and Ralstonia solanacearum.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
David Smith
GBP being Addressed
:
All GBP are produced in recombinant form and labelled with Alexa fluor 488
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands. Identify the ligand(s) that mediate GBP binding. Determine the structures of selected GBPs.