Request ID: cfg_rRequest_2208
Status
:
Approved
Project Description
:
To investigate binding specificities of the 4-binding-site members of the OAA family (OAA-family lectin from the proteobacterium Burkholderia oklahomensis EO14) for comparison to the rest of the OAA family and other anti-HIV lectins
CFG Member
:
Member
Requester First Name
:
Matthew
Requester Last Name
:
Whitley
Head of Lab First Name
:
Angela
Head of Lab Last Name
:
Gronenborn
Assigned First Name
:
Angela M.
Assigned Last Name
:
Gronenborn
Requester Email
:
mwhitley@structbio.pitt.edu
Requester Interest
:
This laboratory focuses on the structural basis of carbohydrate recognition
Request Date [yyyy-mm-dd]
:
2010-11-15
Institution
:
University of Pittsburgh
Shipping Address
:
Department of Structural Biology
University of Pittsburgh
1043 Biomedical Science Tower 3
3501 5th Ave.
Pittsburgh, PA 15260
Comments
:
Information not entered/not applicable.
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2026-06-18
Experiment to be conducted
:
Our laboratory has long been interested in the structural glycobiology of lectins that have potent anti-HIV activity, such as cyanovirin-N . As new families of anti-HIV lectins become known, it is critical that their structures and carbohydrate binding properties be studied systematically. The recently identified OAA family of lectins shows similar anti-HIV activity to other known anti-HIV lectins, but the primary sequences of OAA-family members are distinct from other classes of anti-HIV lectins. Many members of the OAA family contain two carbohydrate binding sites, but some members contain four carbohydrate binding sites. To our knowledge, the structures and binding specificities of the 4-binding-site members of the OAA family have not yet been investigated for comparison to the rest of the OAA family and to other families of anti-HIV lectins. Glycan array screening is requested to support structural studies of the 4-binding-site OAA-family lectin found in the proteobacterium Burkholderia oklahomensis EO147.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Information not entered/not applicable.
GBP being Addressed
:
The protein will be labeled for fluorescent detection using the Alexa Fluor 488 Protein Labeling Kit. Protein can be labeled and shipped to Core H immediately upon approval of this project.
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands. Determine the structures of selected GBPs.