Request ID: cfg_rRequest_2211

Status : Approved
Project Description : Continued studies on influenza A virus HA-glycan interactions by testing another set of mutant HA proteins for their ability to bind sialyated glycans, using recombinant, soluble, trimeric HA preparations
CFG Member : Member
Requester First Name : Xander
Requester Last Name : de Haan
Head of Lab First Name : Peter
Head of Lab Last Name : Rottier
Assigned First Name : Peter P.J.M
Assigned Last Name : Rottier
Requester Email : c.a.m.dehaan@uu.nl
Requester Interest : This laboratory is focusing on virus-host interactions at the cellular and molecular level, with particular focus on influenza- and nidoviruses.
Request Date [yyyy-mm-dd] : 2010-11-18
Institution : Utrecht University
Shipping Address : Information not entered/not applicable.
Comments : Information not entered/not applicable.
CFG Core : H
Resource Type : Glycoarray
Amount Requested : Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd] : 2011-01-15
Experiment to be conducted : We would like to continue our ongoing research on influenza A virus HA-glycan interactions. We would like to test another set of HA (mutant) proteins for their ability to bind sialylated glycans. Again we will use recombinant, soluble, trimeric HA preparations.
Within Scope of Consortium : Y
If yes, indicate the person responsible for inputing data into core B : Xander de Haan
GBP being Addressed : standard protocol using antibodies against the STREP-tag.
Specifc aims being addressed : Define the specificity and affinity for carbohydrate ligands.