Request ID: cfg_rRequest_2261
Status
:
Approved
Project Description
:
continued studies RR#2211 - ongoing research on influenza A virus HA-glycan interactions by testing another set of mutant HA proteins for their ability to bind sialylated glycans
CFG Member
:
Member
Requester First Name
:
Xander
Requester Last Name
:
de Haan
Head of Lab First Name
:
Peter
Head of Lab Last Name
:
Rottier
Assigned First Name
:
Peter P.J.M
Assigned Last Name
:
Rottier
Requester Email
:
c.a.m.dehaan@uu.nl
Requester Interest
:
This laboratory is focusing on virus-host interactions at the cellular and molecular level, with particular focus on influenza- and nidoviruses.
Request Date [yyyy-mm-dd]
:
2011-02-17
Institution
:
Utrecht University
Shipping Address
:
Information not entered/not applicable.
Comments
:
Information not entered/not applicable.
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2026-04-13
Experiment to be conducted
:
We would like to continue our screening of HA-receptor interactions. We would like to repeat the previous request (2211),which mainly focussed on H1, as we think that something must have gone wrong: the signal intensities do not correspond with our fetuin binding assay as usually is the case. Therefore we would like to send some new batches as well as some material for new H1 variants that demonstrate differential binding in our fetuin binding assay (15 samples in total).
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Xander de Haan
GBP being Addressed
:
soluble trimers of HA with strep tag. An HA tree will be made by using monoclonal antibodies to the strep tag and fluorescently labeled anti-mouse antibodies.
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands. Identify the ligand(s) that mediate GBP binding.