Request ID: cfg_rRequest_2323
Status
:
Approved
Project Description
:
To identify all glycans that bind specifically to the FimH lectin domain and to determine the affinities for these carbohydrate ligands
CFG Member
:
Not Member
Requester First Name
:
Henri
Requester Last Name
:
De Greve
Head of Lab First Name
:
Lode
Head of Lab Last Name
:
Wyns
Assigned First Name
:
Lode
Assigned Last Name
:
Wyns
Requester Email
:
hdegreve@vub.ac.be
Requester Interest
:
Information not entered/not applicable.
Request Date [yyyy-mm-dd]
:
2011-04-07
Institution
:
Vrije Universiteit Brussel
Shipping Address
:
Structural Biology Brussels
Building E4
Pleinlaan 2
B-1050 Brussels
Belgium
Tel: +3226291844
Comments
:
Information not entered/not applicable.
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2026-01-21
Experiment to be conducted
:
To identify the carbohydrate-receptor of a putative bacterial adhesin FimH. We cloned and purified the lectin domain of this bacterial adhesin FimH. The lectin domain contain a C-terminal His-tag, used for purification. Objectives for the glycan array experiment: 1) to identify all glycans that bind specifically to the FimH lectin domain, 2) Determine the affinities for these carbohydrate ligands.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Information not entered/not applicable.
GBP being Addressed
:
Information not entered/not applicable.
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands. Identify the ligand(s) that mediate GBP binding.