Request ID: cfg_rRequest_399
Status
:
Approved
Project Description
:
Glycan array screening of the FedF adhesin on F18 fimbriae that mediates adherence on Shiga toxin-producing E. coli strains
CFG Member
:
Not Consortium Member
Requester First Name
:
Julie
Requester Last Name
:
Bouckaert
Head of Lab First Name
:
Lode
Head of Lab Last Name
:
Wyns
Assigned First Name
:
Lode
Assigned Last Name
:
Wyns
Requester Email
:
bouckaej@vub.ac.be
Requester Interest
:
Information not entered/not applicable.
Request Date [yyyy-mm-dd]
:
2005-09-22
Institution
:
Department of Molecular and Cellular Interactions
Vrije Universiteit Brussel (VUB) and
Flanders Interuniversity Institute for Biotechnology (VIB)
Shipping Address
:
Dr. Julie Bouckaert
ULTR, Building E 4.18
Vrije Universiteit Brussel
Pleinlaan 2, 1050 Brussel, Belgium
Tel. 32-2-629-1988, Fax 32-2-6291963
Comments
:
Purified protein is available now, please specify the concentration,volume, if critical also buffer, required
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2026-06-18
Experiment to be conducted
:
F18 fimbriae mediate adherence of Shiga toxin Stx2e-producing E. coli strains, that are the cause of oedema disease and severe neuropathology
in recently-weaned piglets. The affected piglets usually cannot be saved and mortality can reach 90%, leading to large economical losses.
The FedF adhesin at the tip of the flexible F18 fimbriae binds to carbohydrate receptors on the microvilli of the porcine small instestine.
We are in the middle of a project aiming to unravel the structure-function relationship of the FedF adhesin and succeeded in cloning,
expression and purification of the lectin domain of FedF. Surface plasmon resonance measurements on a Biacore3000 could however not confirm
the binding to hypothetical carbohydrate ligands (Vet. Microbiol. 2004 Jun 3;100(3-4):241-6: Inhibition of adhesion of F18+ Escherichia coli
to piglet intestinal villous enterocytes by monoclonal antibody against blood group H-2 antigen). However, mucin from porcine intestine
appears to contain the inhibitory glycans.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Dr. Ola Blixt
GBP being Addressed
:
FedF
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands. Establish the cell types involved in communication. Identify the ligand(s) that mediate GBP binding. Determine how GBP-ligand interactions mediate cell communication. Determine the structures of selected GBPs. Identify the glycosyltransferases that synthesize carbohydrate ligand(s). Determine whether regulation of glycosylation modulates GBP function.