Request ID: cfg_rRequest_413
Status
:
Approved
Project Description
:
Request is for glycan array slides to test freshly isolated transfected and normal human leukocyte subtypes expressing various forms of Siglec-8.
CFG Member
:
Siglec
Requester First Name
:
Bruce
Requester Last Name
:
Bochner
Head of Lab First Name
:
Bruce
Head of Lab Last Name
:
Bochner
Assigned First Name
:
Bruce S.
Assigned Last Name
:
Bochner
Requester Email
:
bbochner@jhmi.edu
Requester Interest
:
This laboratory focuses on the role of cell adhesion molecules in allergic inflammation. One main area of ongoing research is examining the function, expression and ligands for Siglec-8 and Siglec-F on eosinophils and Siglec-8 on mast cells.
Request Date [yyyy-mm-dd]
:
2005-10-21
Institution
:
Johns Hopkins University School of Medicine
Shipping Address
:
Johns Hopkins Asthma and Allergy Center
5501 Hopkins Bayview Circle, Room 2B71
Baltimore, MD 21224
Comments
:
I will collaborate with Ron Schnaar, also here at Johns Hopkins, using his published method for intact cell adhesion to slides (Nimrichter et al. (2004) Glycobiology 14, 197) that is best executed using freshly isolated cells and custom adhesion equipment on hand in Baltimore.
I'm not quite sure how many slides to request -- are they reusable? We might need to fix the cells in place. If so, maybe 4 slides to permit replicate analyses with more than one cell type? Might need more later.
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2025-03-25
Experiment to be conducted
:
Our plan is to follow up on the previous Siglec-8 ligand consortium work by using intact transfected and purified normal human leukocyte subtypes expressing various forms of Siglec-8 to verify ligand and to screen for other ligands.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Bruce Bochner
GBP being Addressed
:
Siglec-8 ligands
Specifc aims being addressed
:
Establish the cell types involved in communication. Identify the ligand(s) that mediate GBP binding.