Request ID: cfg_rRequest_458
Status
:
Approved
Project Description
:
Glycan array screening of a set of soluble lectins from opportunistic bacteria
CFG Member
:
Other
Requester First Name
:
Anne
Requester Last Name
:
Imberty
Head of Lab First Name
:
Anne
Head of Lab Last Name
:
Imberty
Assigned First Name
:
Anne
Assigned Last Name
:
Imberty
Requester Email
:
imberty@cermav.cnrs.fr
Requester Interest
:
This laboratory focuses on structural and thermodynamical characterization of lectin-carbohydrate interactions.
Request Date [yyyy-mm-dd]
:
2005-12-13
Institution
:
CERMAV-CNRS
Shipping Address
:
Information not entered/not applicable.
Comments
:
Contact has been established with Rick Alvarez who has been helpful in describing protocols that we will have to use to label our lectins. He also suggested that we propose several lectins (up to 10) rather than one or two. Of course, we like this idea, since we have lectins from several bacteria and it will be useful to compare their affinity. Nevertheless, if the required number is too high, we can make a priority list.
Each of the lectin is produced in its recombinant form and we are now in the process of labelling them with Alexa 488.
CFG Core
:
H
Resource Type
:
Glycoarray
Amount Requested
:
Information not entered/not applicable.
Date that your RNA/GBP samples will be sent to the core [yyyy-mm-dd]
:
2007-11-01
Experiment to be conducted
:
The aim of the experiment is to determine the fine specificiy of a series of soluble lectins from opportunistic bacteria. These include Pseudomonas aeruginosa (2 lectins PAIL and PA-IIL) and Burkholderia cenocepacia (BclA related to PA-IIL) that cause deadly infection in immunocompromised patients and are also highly dangerous for cystic fibrosis patients. Chromobacterium violaceum (CV-IIL related to PA-IIL) is of biotechnological interest but is also able to create fatal septicemy in humans. Finally Ralstonia solanacearum is a plant pathogen but it can also infect mammals and contain several mannose and fucose binding lectins of interest (RS-IIL related to PA-IIL, RSL and RS20L).
All of these soluble lectins have been proven to, or are supposed to, play a role in host recognition, in infection and/in biofilm formation. The identification of highest affinity glycans will be helpful for i/ identifying the target on human tissues and ii/ designing glycomimics that could compete with the bacteria and serve as anti-adhesive compounds.
Within Scope of Consortium
:
Y
If yes, indicate the person responsible for inputing data into core B
:
Anne Imberty
GBP being Addressed
:
PA-IL : galactose-binding lectin from Pseudomonas aeruginosa (tetramer)
PA-IIL : fucose-binding lectin from Pseudomonas aeruginosa (tetramer)
CV-IIL : fucose/mannose binding lectin from Cromobacterium violaceum (tetramer)
BclA : mannose-binding lectin from Burkholderia cenocepacia (dimer)
RS-IIL : mannose-binding lectin from Ralstonia solanacearum (tetramer)
RSL : fucose binding lectin from Ralstonia solanacearum (dimer with 5 sites/monomer)
RS20L : mannose-binding lectin from Ralstonia solanacearum (trimer)
Specifc aims being addressed
:
Define the specificity and affinity for carbohydrate ligands. Identify the ligand(s) that mediate GBP binding. Determine how GBP-ligand interactions mediate cell communication. Determine the structures of selected GBPs.